The effect of temperature of cardioplegic solution on myocardial preservation was studied using isolated rat heart perfusion technique. Twenty Sprague-Dawley rats, weighing 120-140gm, were pretreated with intraperitoneal injection of heparin
sodium(300u/kg) and then the hearts were excised after cervical herniation 30 minutes later. The hearts were perfused in isolated working heart apparatus with oxygenated modified Tyrode solution at 37℃. After 10 minutes of non working heart
perfusion,
the hearts were subjected to arrest for 30 minutes by administration of 5cc cardioplegic solution at the temperature of 4℃(Group I), 15℃(Group II), 25℃(Group III), 37℃(Group Ⅳ). At the same time, the topical cooling of heart was performed
using ice
saline. After arrest, the hearts were reperfused by non working heart perfusion for 1 hour with modified Throde solution at 37%. The CPK, GOT and LDH in reperfusate were measured at 5,20,40,60 minutes after start of reperfusion. With the values
of
those, we compared the effect of temperature of cardioplegic solution on myocardial preservation.
@ES The results were as follows;
@EN 1. The enzyme values in reperfusate were highest at 5 minute and after then declined.
2. At 5 minutes after reperfusion, the enzyme values in Group I were lower than those in other Groups.
These results suggest that the cardioplegic solutions using for cardiac arrest and myocardial protection can be working better at 4℃ than at any other temperature.
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